Peptide Synthesis and Applications
John Howl
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
Categories:
Year:
2005
Edition:
1
Publisher:
Humana Press
Language:
english
Pages:
273
ISBN 10:
1592598773
ISBN 13:
9781592598779
Series:
Methods in Molecular Biology
File:
PDF, 7.01 MB
IPFS:
,
english, 2005
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