Membrane Protein Protocols
Barry S. Selinsky
A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
Categories:
Year:
2010
Edition:
1st Edition.
Publisher:
Humana Press
Language:
english
Pages:
307
ISBN 10:
1617373761
ISBN 13:
9781617373763
Series:
Methods in Molecular Biology
File:
PDF, 3.03 MB
IPFS:
,
english, 2010