Cell-Free Gene Expression: Methods and Protocols

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Biology and other natural sciences - Molecular
Cell-Free Gene Expression: Methods and...

Cell-Free Gene Expression: Methods and Protocols

Name: Cell-Free Gene Expression: Methods and Protocols
ISBN: 9781071619971

Ashty S. Karim, Michael C. Jewett

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This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

Categories:

Biology and other natural sciences - Molecular

Year:

2022

Publisher:

Humana

Language:

english

Pages:

450

ISBN 10:

1071619977